Summary
FastProof™ SARS-CoV-2 RT-LAMP Kit
(Cat. No. ZE-P10)
(For research use only)
FastProof™ SARS-CoV-2 RT-LAMP Kit used Colorimetric RT-LAMP (Reverse-Transcription Loop-Mediated Isothermal Amplification) technique, using a primer set (6 primers) specific for the target gene, allows the highly specific detection of SARS-CoV-2.
Reagents Supplied
Components Included within the kit
| Reagents | Cat. No. | Size |
|---|---|---|
| Colorimetric RT-LAMP master mix | ZE-P02 | 1,056 μl |
| RdRp primers mix | ZE-P04 | 224 μl |
| N primers mix | ZE-P05 | 224 μl |
| RNase P primers mix | ZE-P06 | 224 μl |
| Positive control | ZE-P07 | 224 μl |
| Negative control | ZE-P08 | 224 μl |
Brief Protocol
1. Thaw all components at room temperature including colorimetric RT-LAMP master mix, primer mix, positive control, and negative control (RNase-free water) after that place on ice.
2. Preparation of RT-LAMP Supermix for RT-LAMP Reactions. Mix the colorimetric RT-LAMP master mix (pink solution) with the primer mix as described in Table. (Carry out in a biosafety cabinet, a laminar flow cabinet, or a clean closed-system cabinet such as a PCR cabinet workstation)
| Reagents | Size |
|---|---|
| Colorimetric RT-LAMP master mix | 11 μl |
| Primers mix (of each target genes) | 4 μl |
| Total volume | 15 μl |
3. Aliquot 15 μl of the RT-LAMP supermix into each of the PCR tubes. Add 5 μl of the extracted RNA sample to the reaction PCR tube and mix well by pipetting up and down gently.
4. Incubate the reactions at room temperature for 10 minutes or incubate at 37°C for 10 minutes and incubate at 65°C for 50 minutes in a Heating block. Before result interpretation, keep the reaction cold for 1 minute.
5. Remove PCR tube from incubator and result is observed by the naked eye of color change.
Performance Report
Validation of colorimetric RT-LAMP on clinical samples
Manual Download
SARS-CoV-2 RT-LAMP Kit
Troubleshooting Guide
Precipitation of the master mix after repeated freeze/thaw cycles
- Vortex or invert the tube several times until the precipitates disappear.
Negative control turns yellow (False positive)
- Clean your working space and equipment.
- Change RNase free water.
- Check the temperature control system of the thermal cycler machine.
- Do not let the master mix and the reaction expose to air for too long by leaving the lid open during preparation of the master mix and the reaction.
- Always keep the master mix and the reaction at 4°C.
Zenostic 