Summary

FastProof™ SARS-CoV-2 RT-LAMP Kit
(Cat. No. ZE-P10)
(For research use only)

FastProof™ SARS-CoV-2 RT-LAMP Kit used Colorimetric RT-LAMP (Reverse-Transcription Loop-Mediated Isothermal Amplification) technique, using a primer set (6 primers) specific for the target gene, allows the highly specific detection of SARS-CoV-2.

Reagents Supplied

Components Included within the kit

ReagentsCat. No.Size
Colorimetric RT-LAMP master mixZE-P021,056 μl
RdRp primers mixZE-P04224 μl
N primers mixZE-P05224 μl
RNase P primers mixZE-P06224 μl
Positive controlZE-P07224 μl
Negative controlZE-P08224 μl

Brief Protocol

1. Thaw all components at room temperature including colorimetric RT-LAMP master mix, primer mix, positive control, and negative control (RNase-free water) after that place on ice.

2. Preparation of RT-LAMP Supermix for RT-LAMP Reactions. Mix the colorimetric RT-LAMP master mix (pink solution) with the primer mix as described in Table. (Carry out in a biosafety cabinet, a laminar flow cabinet, or a clean closed-system cabinet such as a PCR cabinet workstation)

ReagentsSize
Colorimetric RT-LAMP master mix11 μl
Primers mix (of each target genes)4 μl
Total volume15 μl

3. Aliquot 15 μl of the RT-LAMP supermix into each of the PCR tubes. Add 5 μl of the extracted RNA sample to the reaction PCR tube and mix well by pipetting up and down gently.

4. Incubate the reactions at room temperature for 10 minutes or incubate at 37°C for 10 minutes and incubate at 65°C for 50 minutes in a Heating block. Before result interpretation, keep the reaction cold for 1 minute.

5. Remove PCR tube from incubator and result is observed by the naked eye of color change.

Performance Report

Validation of colorimetric RT-LAMP on clinical samples

Manual Download

SARS-CoV-2 RT-LAMP Kit
Troubleshooting Guide

Precipitation of the master mix after repeated freeze/thaw cycles

  • Vortex or invert the tube several times until the precipitates disappear.

Negative control turns yellow (False positive)

  • Clean your working space and equipment.
  • Change RNase free water.
  • Check the temperature control system of the thermal cycler machine.
  • Do not let the master mix and the reaction expose to air for too long by leaving the lid open during preparation of the master mix and the reaction.
  • Always keep the master mix and the reaction at 4°C.